Conditions for protoplasting, regenerating, and transforming the calicheamicin producer, Micromonospora echinospora.
We report methods to generate protoplasts, to regenerate mycelia, and to transform Micromonospora echinospora. This actinomycete produces the unusual antitumor antibiotics, the calicheamicins. These protocols may be applied to other actinomycetes that have been difficult to transform. These methods...
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| Udgivet i: | Appl Environ Microbiol |
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| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1992
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC195603/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1599256/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.58.4.1376-1378.1992 |
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