Conditions for protoplasting, regenerating, and transforming the calicheamicin producer, Micromonospora echinospora.

We report methods to generate protoplasts, to regenerate mycelia, and to transform Micromonospora echinospora. This actinomycete produces the unusual antitumor antibiotics, the calicheamicins. These protocols may be applied to other actinomycetes that have been difficult to transform. These methods...

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Publié dans:	Appl Environ Microbiol
Auteurs principaux:	Love, S F, Maiese, W M, Rothstein, D M
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1992
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC195603/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1599256/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.58.4.1376-1378.1992
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