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High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae

Eukaryotic membrane proteins are often difficult to produce in large quantities, which is a significant obstacle for further structural and biochemical investigation. Based on the analysis of 43 eukaryotic membrane proteins, we present a cost-effective high-throughput approach for rapidly screening...

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Main Authors: Newstead, Simon, Kim, Hyun, von Heijne, Gunnar, Iwata, So, Drew, David
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 2007
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1955786/
https://ncbi.nlm.nih.gov/pubmed/17709746
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0704546104
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