Detection of low numbers of bacterial cells in soils and sediments by polymerase chain reaction.
Polymerase chain reaction was used to amplify the low copy number of two 16S ribosomal gene fragments from soil and sediment extracts. Total DNA for polymerase chain reaction was extracted from 1 g of seeded or unseeded samples by a rapid freeze-and-thaw method. Amplified DNA fragments can be detect...
Enregistré dans:
| Publié dans: | Appl Environ Microbiol |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology (ASM)
1992
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC195321/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1610201/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.58.2.754-757.1992 |
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