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Expression, purification, crystallization and preliminary X-ray crystallographic analysis of pantothenate kinase from Mycobacterium tuberculosis

Pantothenate kinase is an essential enzyme in the bacterial life cycle. It catalyzes the phosphorylation of pantothenate (vitamin B(5)) to 4′-phosphopantothenate, the first step in the coenzyme A biosynthetic pathway. The enzyme from Mycobacterium tuberculosis, MW 35.7 kDa, has been cloned, expresse...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Das, Satyabrata, Kumar, Parimal, Bhor, Vikrant, Surolia, A., Vijayan, M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: International Union of Crystallography 2004
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952411/
https://ncbi.nlm.nih.gov/pubmed/16508093
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309104028040
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