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Expression, purification, crystallization and preliminary X-ray crystallographic studies of a cold-adapted aspartate carbamoyltransferase from Moritella profunda

Aspartate carbamoyltransferase (ATCase) catalyzes the carbamoylation of the α-amino group of l-aspartate by carbamoyl phosphate (CP) to yield N-­carbamoyl-l-aspartate and orthophosphate in the first step of de novo pyrimidine biosynthesis. Apart from its key role in nucleotide metabolism, the enzyme...

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Detalhes bibliográficos
Main Authors: De Vos, Dirk, Hulpiau, Paco, Vergauwen, Bjorn, Savvides, Savvas N., Van Beeumen, Jozef
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952289/
https://ncbi.nlm.nih.gov/pubmed/16511017
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910500285X
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