Expression, purification, crystallization and preliminary crystallographic analysis of a stand-alone RAM domain with hydrolytic activity from the hyperthermophile Pyrococcus furiosus

The RAM domain is one of several ligand-binding modules present in prokaryotes that are presumed to regulate the transcription of specific genes. To date, no hydrolytic activity has been reported for such modules. Curiously, a stand-alone RAM domain in Pyrococcus furiosus was isolated during a scree...

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Autori principali: Agapay, Ramelito C., Savvides, Savvas N., Van Driessche, Gonzalez, Devreese, Bart, Van Beeumen, Jozef, Jongejan, Jaap A., Hagen, Wilfred R.
Natura: Artigo
Lingua:Inglês
Pubblicazione: International Union of Crystallography 2005
Soggetti:
Crystallization Communications
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1991312/
https://ncbi.nlm.nih.gov/pubmed/16511194
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309105028393
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