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Expression, purification, crystallization and preliminary X-ray analysis of sepiapterin reductase from Chlorobium tepidum

Sepiapterin reductase from Chlorobium tepidum (CT-SR) produces l-threo-tetrahydrobiopterin, an isomer of tetrahydrobiopterin, in the last step of de novo synthesis initiating from GTP. Native CT-SR and a selenomethionine (SeMet) derivative of CT-SR have been crystallized by the hanging-drop vapour-d...

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Main Authors: Supangat, Supangat, Choi, Yong Kee, Park, Young Shik, Son, Daeyoung, Han, Chang-deok, Lee, Kon Ho
Formáid: Artigo
Teanga:Inglês
Foilsithe: International Union of Crystallography 2005
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952253/
https://ncbi.nlm.nih.gov/pubmed/16510994
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910403444X
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