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Expression, purification, crystallization and preliminary X-ray analysis of sepiapterin reductase from Chlorobium tepidum
Sepiapterin reductase from Chlorobium tepidum (CT-SR) produces l-threo-tetrahydrobiopterin, an isomer of tetrahydrobiopterin, in the last step of de novo synthesis initiating from GTP. Native CT-SR and a selenomethionine (SeMet) derivative of CT-SR have been crystallized by the hanging-drop vapour-d...
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| Hauptverfasser: | , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
International Union of Crystallography
2005
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1952253/ https://ncbi.nlm.nih.gov/pubmed/16510994 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910403444X |
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