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Ty1 reverse transcriptase does not read through the proposed 2′,5′-branched retrotransposition intermediate in vitro
2′,5′-Branched RNA was recently proposed as a key Ty1 retrotransposition intermediate, for which cleavage by lariat debranching enzyme (Dbr1p) enables reverse transcription to continue synthesizing the complete Ty1 cDNA. Because dbr1 cells can produce substantial Ty1 cDNA despite lacking Dbr1p, the...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2007
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1950764/ https://ncbi.nlm.nih.gov/pubmed/17652136 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.629607 |
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