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Influence of C-5 halogenation of uridines on hairpin versus duplex RNA folding

Halogenation of bases is a widespread method used for solving crystal structures of nucleic acids. However, this modification may have important consequences on RNA folding and thus on the success of crystallization. We have used a combination of UV thermal melting, steady-state fluorescence, X-ray...

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Detalhes bibliográficos
Main Authors: Ennifar, Eric, Bernacchi, Serena, Wolff, Philippe, Dumas, Philippe
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1950758/
https://ncbi.nlm.nih.gov/pubmed/17630326
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.408507
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