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Domain selection combined with improved cloning strategy for high throughput expression of higher eukaryotic proteins
BACKGROUND: Expression of higher eukaryotic genes as soluble, stable recombinant proteins is still a bottleneck step in biochemical and structural studies of novel proteins today. Correct identification of stable domains/fragments within the open reading frame (ORF), combined with proper cloning str...
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| Auteurs principaux: | , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
BioMed Central
2007
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1950093/ https://ncbi.nlm.nih.gov/pubmed/17663785 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-7-45 |
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