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Single-cell zeroth-order protein degradation enhances the robustness of synthetic oscillator

In Escherichia coli, protein degradation in synthetic circuits is commonly achieved by the ssrA-tagged degradation system. In this work, we show that the degradation kinetics for the green fluorescent protein fused with the native ssrA tag in each cell exhibits the zeroth-order limit of the Michaeli...

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Bibliografiske detaljer
Main Authors: Wong, Wilson W, Tsai, Tony Y, Liao, James C
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2007
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1943427/
https://ncbi.nlm.nih.gov/pubmed/17667952
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/msb4100172
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