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A Combined Atomic Force/Fluorescence Microscopy Technique to Select Aptamers in a Single Cycle From a Small Pool of Random Oligonucleotides
We develop a method, which utilizes a combined atomic force microscope (AFM)/fluorescence microscope and small copy number polymerase chain reaction (PCR), to affinity-select individual aptamer species in a single cycle from a small pool of random-sequence oligonucleotides (oligos). In this method,...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1939975/ https://ncbi.nlm.nih.gov/pubmed/17262788 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jemt.20421 |
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