Use of Loop-Mediated Isothermal Amplification of the IS900 Sequence for Rapid Detection of Cultured Mycobacterium avium subsp. paratuberculosis

We evaluated the usefulness of loop-mediated isothermal amplification (LAMP) in detecting specific gene sequences of Mycobacterium avium subsp. paratuberculosis (MAP). A total of 102 primer sets for LAMP was designed to amplify the IS900, HspX, and F57 gene sequences of MAP. Using each of two primer...

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Publié dans:	J Clin Microbiol
Auteurs principaux:	Enosawa, M., Kageyama, S., Sawai, K., Watanabe, K., Notomi, T., Onoe, S., Mori, Y., Yokomizo, Y.
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 2003
Sujets:	Mycobacteriology and Aerobic Actinomycetes
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC193777/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12958269/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.41.9.4359-4365.2003
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