Use of Loop-Mediated Isothermal Amplification of the IS900 Sequence for Rapid Detection of Cultured Mycobacterium avium subsp. paratuberculosis

We evaluated the usefulness of loop-mediated isothermal amplification (LAMP) in detecting specific gene sequences of Mycobacterium avium subsp. paratuberculosis (MAP). A total of 102 primer sets for LAMP was designed to amplify the IS900, HspX, and F57 gene sequences of MAP. Using each of two primer...

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Bibliografiske detaljer
Main Authors: Enosawa, M., Kageyama, S., Sawai, K., Watanabe, K., Notomi, T., Onoe, S., Mori, Y., Yokomizo, Y.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2003
Fag:
Mycobacteriology and Aerobic Actinomycetes
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC193777/
https://ncbi.nlm.nih.gov/pubmed/12958269
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.9.4359-4365.2003
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