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DNA Topoisomerase III from the Hyperthermophilic Archaeon Sulfolobus solfataricus with Specific DNA Cleavage Activity

We report the production, purification, and characterization of a type IA DNA topoisomerase, previously designated topoisomerase I, from the hyperthermophilic archaeon Sulfolobus solfataricus. The protein was capable of relaxing negatively supercoiled DNA at 75°C in the presence of Mg(2+). Mutation...

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Detalhes bibliográficos
Main Authors: Dai, Penggao, Wang, Ying, Ye, Risheng, Chen, Liang, Huang, Li
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC193750/
https://ncbi.nlm.nih.gov/pubmed/12949102
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.185.18.5500-5507.2003
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