Noninducible Tet repressor mutations map from the operator binding motif to the C terminus.
We have developed a new genetic selection system for Tet repressor mutations with a noninducible phenotype for tetracycline (TetRs). Extensive chemical mutagenesis of tetR yielded 93 single-site Tet repressor mutations. They map from residue 23 preceding the alpha-helix-turn-alpha-helix operator bin...
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| Publicado no: | J Bacteriol |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1993
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC193041/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8432715/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.175.4.1206-1210.1993 |
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