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Noninducible Tet repressor mutations map from the operator binding motif to the C terminus.

We have developed a new genetic selection system for Tet repressor mutations with a noninducible phenotype for tetracycline (TetRs). Extensive chemical mutagenesis of tetR yielded 93 single-site Tet repressor mutations. They map from residue 23 preceding the alpha-helix-turn-alpha-helix operator bin...

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Bibliografiske detaljer
Udgivet i:J Bacteriol
Principais autores: Hecht, B, Müller, G, Hillen, W
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1993
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC193041/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8432715/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.175.4.1206-1210.1993
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