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Sequence-specific ligation of DNA using RecA protein

A method is described that allows the sequence-specific ligation of DNA. The method is based on the ability of RecA protein from Escherichia coli to selectively pair oligonucleotides to their homologous sequences at the ends of fragments of duplex DNA. These three-stranded complexes were protected f...

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Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Ferrin, Lance J., Camerini-Otero, R. Daniel
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1998
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC19280/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9482854/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.5.2152
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