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Sequence-specific ligation of DNA using RecA protein

A method is described that allows the sequence-specific ligation of DNA. The method is based on the ability of RecA protein from Escherichia coli to selectively pair oligonucleotides to their homologous sequences at the ends of fragments of duplex DNA. These three-stranded complexes were protected f...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Ferrin, Lance J., Camerini-Otero, R. Daniel
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 1998
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC19280/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9482854/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.5.2152
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