A more efficient RNAi inducible system for tight regulation of gene expression in mammalian cells and xenograft animals

Two types of tetracycline-controlled inducible RNAi expression systems have been developed that generally utilize multiple tetracycline operators (TetOs) or repressor fusion proteins to overcome the siRNA leakiness. Here, we report a novel system that overexpresses the tetracycline repressor (TetR)...

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Detalhes bibliográficos
Main Authors: Zhang, Jing, Wang, Cuiying, Ke, Ning, Bliesath, Josh, Chionis, John, He, Qiuchen S., Li, Qi-xiang, Chatterton, Jon E., Wong-staal, Flossie, Zhou, Demin
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2007
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1924899/
https://ncbi.nlm.nih.gov/pubmed/17616554
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.520707
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