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Regulation of DNA nucleases by molecular crowding

Here, we examined the effects of molecular crowding on the function, structure and stability of nucleases. We found that the hydrolysis of a 29-mer double-stranded DNA by the endonucleases DNase I and S1 nuclease was substantially enhanced by molecular crowding using polyethylene glycol (PEG); howev...

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Autors principals: Sasaki, Yoshiharu, Miyoshi, Daisuke, Sugimoto, Naoki
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2007
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1919490/
https://ncbi.nlm.nih.gov/pubmed/17567601
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm445
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