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Expression of the human papillomavirus type 11 L1 capsid protein in Escherichia coli: characterization of protein domains involved in DNA binding and capsid assembly.

The L1 major capsid protein of human papillomavirus type 11 (HPV-11) was expressed in Escherichia coli, and the soluble recombinant protein was purified to near homogeneity. The recombinant L1 protein bound DNA as determined by the Southwestern assay method, and recombinant mutant L1 proteins locali...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:J Virol
Hauptverfasser: Li, M, Cripe, T P, Estes, P A, Lyon, M K, Rose, R C, Garcea, R L
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1997
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC191427/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9060658/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.71.4.2988-2995.1997
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