Expression of the polyomavirus minor capsid proteins VP2 and VP3 in Escherichia coli: in vitro interactions with recombinant VP1 capsomeres.

The polyomavirus VP2 and VP3 capsid proteins were expressed in Escherichia coli. The majority of the expressed proteins were in an insoluble fraction, and they were extracted and initially purified in 8 M urea before renaturation. Soluble VP2 and VP3 were mixed with purified recombinant VP1 capsomer...

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Detalhes bibliográficos

Publicado no:	J Virol
Principais autores:	Delos, S E, Cripe, T P, Leavitt, A D, Greisman, H, Garcea, R L
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC189715/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7494283/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.69.12.7734-7742.1995
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