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Real-Time Measurement of Myosin–Nucleotide Noncovalent Complexes by Electrospray Ionization Mass Spectrometry
Nanoelectrospray ionization mass spectrometry has been used to measure the binding of ATP and ADP to the active site of rabbit skeletal myosin-S1. Increases in the molecular mass of myosin-S1 of 425 ± 10 Da were obtained with the binding of ADP to the active site and by 530 ± 10 Da with either ATP o...
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| Autori principali: | , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
The Biophysical Society
2007
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1913167/ https://ncbi.nlm.nih.gov/pubmed/17483158 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.101618 |
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