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Real-Time Measurement of Myosin–Nucleotide Noncovalent Complexes by Electrospray Ionization Mass Spectrometry

Nanoelectrospray ionization mass spectrometry has been used to measure the binding of ATP and ADP to the active site of rabbit skeletal myosin-S1. Increases in the molecular mass of myosin-S1 of 425 ± 10 Da were obtained with the binding of ADP to the active site and by 530 ± 10 Da with either ATP o...

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Detalhes bibliográficos
Main Authors: White, Howard D., Ashcroft, Alison E.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1913167/
https://ncbi.nlm.nih.gov/pubmed/17483158
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.101618
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