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Monitoring conformational dynamics of a single molecule by selective fluorescence spectroscopy

A recently developed, real-time spectroscopic technique, burst-integrated fluorescence lifetime (BIFL), is shown to be well suited for monitoring the individual molecular conformational dynamics of a single molecule diffusing through the microscopic, open measurement volume (≈10 fl) of a confocal ep...

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Auteurs principaux: Eggeling, C., Fries, J. R., Brand, L., Günther, R., Seidel, C. A. M.
Format: Artigo
Langue:Inglês
Publié: The National Academy of Sciences 1998
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC19090/
https://ncbi.nlm.nih.gov/pubmed/9465054
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