Activity of purified hepatitis C virus protease NS3 on peptide substrates.

The protease domain of the hepatitis C virus (HCV) protein NS3 was expressed in Escherichia coli, purified to homogeneity, and shown to be active on peptides derived from the sequence of the NS4A-NS4B junction. Experiments were carried out to optimize protease activity. Buffer requirements included...

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Detalhes bibliográficos

Publicado no:	J Virol
Principais autores:	Steinkühler, C, Urbani, A, Tomei, L, Biasiol, G, Sardana, M, Bianchi, E, Pessi, A, De Francesco, R
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1996
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC190711/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8794305/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.70.10.6694-6700.1996
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