Defining Blood Processing Parameters for Optimal Detection of Cryopreserved Antigen-Specific Responses for HIV Vaccine Trials

Interferon-gamma (IFN-γ) ELISpot and intracellular cytokine staining (ICS) assays are routinely employed in clinical HIV vaccine trials to identify antigen-specific T cells in cryopreserved peripheral blood mononuclear cells (PBMC). Several parameters involved in blood collection, processing and shi...

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Detalhes bibliográficos
Main Authors: Bull, Marta, Lee, Deborah, Stucky, Jason, Chiu, Ya-Lin, Rubin, Abbe, Horton, Helen, McElrath, M. Juliana
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1904432/
https://ncbi.nlm.nih.gov/pubmed/17382342
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2007.02.003
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