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Defining Blood Processing Parameters for Optimal Detection of Cryopreserved Antigen-Specific Responses for HIV Vaccine Trials
Interferon-gamma (IFN-γ) ELISpot and intracellular cytokine staining (ICS) assays are routinely employed in clinical HIV vaccine trials to identify antigen-specific T cells in cryopreserved peripheral blood mononuclear cells (PBMC). Several parameters involved in blood collection, processing and shi...
Bewaard in:
Hoofdauteurs: | , , , , , , |
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Formaat: | Artigo |
Taal: | Inglês |
Gepubliceerd in: |
2007
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Onderwerpen: | |
Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1904432/ https://ncbi.nlm.nih.gov/pubmed/17382342 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2007.02.003 |
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