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Amino acid residues of the Escherichia coli tRNA(m(5)U54)methyltransferase (TrmA) critical for stability, covalent binding of tRNA and enzymatic activity

The Escherichia coli trmA gene encodes the tRNA(m(5)U54)methyltransferase, which catalyses the formation of m(5)U54 in tRNA. During the synthesis of m(5)U54, a covalent 62-kDa TrmA-tRNA intermediate is formed between the amino acid C324 of the enzyme and the 6-carbon of uracil. We have analysed the...

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Bibliografske podrobnosti
Main Authors: Urbonavičius, Jaunius, Jäger, Gunilla, Björk, Glenn R.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2007
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1904294/
https://ncbi.nlm.nih.gov/pubmed/17459887
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm205
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