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Evidence that the nuclease activities associated with the herpes simplex type 1 DNA polymerase are due to the 3'-5' exonuclease.

We investigated nuclease activities associated with the catalytic subunit of herpes simplex virus type 1 DNA polymerase. We confirm that a 3'-5' exonuclease copurifies with this enzyme. Previous reports suggested that a 5' DNase was intrinsic to the polymerase. Our preparation lacks such activity.

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Udgivet i:J Virol
Principais autores: Hall, J D, Orth, K L, Claus-Walker, D
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1996
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC190424/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8676514/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.70.7.4816-4818.1996
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