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DNA-binding domains of Fos and Jun do not induce DNA curvature: An investigation with solution and gel methods

We demonstrate the use of a DNA minicircle competition binding assay, together with DNA cyclization kinetics and gel-phasing methods, to show that the DNA-binding domains (dbd) of the heterodimeric leucine zipper protein Fos–Jun do not bend the AP-1 target site. Our DNA constructs contain an AP-1 si...

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Bibliografiske detaljer
Main Authors: Sitlani, Ayesha, Crothers, Donald M.
Format: Artigo
Sprog:Inglês
Udgivet: The National Academy of Sciences 1998
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC19021/
https://ncbi.nlm.nih.gov/pubmed/9465027
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