DNA-binding domains of Fos and Jun do not induce DNA curvature: An investigation with solution and gel methods
We demonstrate the use of a DNA minicircle competition binding assay, together with DNA cyclization kinetics and gel-phasing methods, to show that the DNA-binding domains (dbd) of the heterodimeric leucine zipper protein Fos–Jun do not bend the AP-1 target site. Our DNA constructs contain an AP-1 si...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1998
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC19021/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9465027/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.4.1404 |
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