Defined large-scale alterations of the human cytomegalovirus genome constructed by cotransfection of overlapping cosmids.
We have constructed defined human cytomegalovirus (CMV) mutants by cotransfecting overlapping cosmid clones spanning the 230-kbp genome. Using this strategy, we have introduced a 13-kbp region of DNA from a virulent strain of CMV into a defined position within the avirulent CMV(Towne) genome. Althou...
Guardat en:
| Publicat a: | J Virol |
|---|---|
| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1996
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC190037/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8627734/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.70.3.2044-2048.1996 |
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