Defined large-scale alterations of the human cytomegalovirus genome constructed by cotransfection of overlapping cosmids.

We have constructed defined human cytomegalovirus (CMV) mutants by cotransfecting overlapping cosmid clones spanning the 230-kbp genome. Using this strategy, we have introduced a 13-kbp region of DNA from a virulent strain of CMV into a defined position within the avirulent CMV(Towne) genome. Althou...

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izdano v:	J Virol
Principais autores:	Kemble, G, Duke, G, Winter, R, Spaete, R
Format:	Artigo
Jezik:	Inglês
Izdano:	American Society for Microbiology (ASM) 1996
Teme:	Research Article
Online dostop:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC190037/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8627734/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.70.3.2044-2048.1996
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