Maternal germ-line transmission of mutant mtDNAs from embryonic stem cell-derived chimeric mice

We report a method for introducing mtDNA mutations into the mouse female germ line by means of embryonic stem (ES) cell cybrids. Mitochondria were recovered from the brain of a NZB mouse by fusion of synaptosomes to a mtDNA-deficient (ρ°) cell line. These cybrids were enucleated and the cytoplasts w...

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Publié dans:	Proc Natl Acad Sci U S A
Auteurs principaux:	Sligh, James E., Levy, Shawn E., Waymire, Katrina G., Allard, Paulette, Dillehay, Dirck L., Nusinowitz, Steven, Heckenlively, John R., MacGregor, Grant R., Wallace, Douglas C.
Format:	Artigo
Langue:	Inglês
Publié:	National Academy of Sciences 2000
Sujets:	Biological Sciences
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC18941/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11106380/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.250491597
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