Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry

We present a strategy for the analysis of the yeast phosphoproteome that uses endo-Lys C as the proteolytic enzyme, immobilized metal affinity chromatography for phosphopeptide enrichment, a 90-min nanoflow-HPLC/electrospray-ionization MS/MS experiment for phosphopeptide fractionation and detection,...

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Main Authors: Chi, An, Huttenhower, Curtis, Geer, Lewis Y., Coon, Joshua J., Syka, John E. P., Bai, Dina L., Shabanowitz, Jeffrey, Burke, Daniel J., Troyanskaya, Olga G., Hunt, Donald F.
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 2007
Teme:
Physical Sciences
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1892997/
https://ncbi.nlm.nih.gov/pubmed/17287358
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0607084104
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