Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry

We present a strategy for the analysis of the yeast phosphoproteome that uses endo-Lys C as the proteolytic enzyme, immobilized metal affinity chromatography for phosphopeptide enrichment, a 90-min nanoflow-HPLC/electrospray-ionization MS/MS experiment for phosphopeptide fractionation and detection,...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Chi, An, Huttenhower, Curtis, Geer, Lewis Y., Coon, Joshua J., Syka, John E. P., Bai, Dina L., Shabanowitz, Jeffrey, Burke, Daniel J., Troyanskaya, Olga G., Hunt, Donald F.
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2007
Schlagworte:
Physical Sciences
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1892997/
https://ncbi.nlm.nih.gov/pubmed/17287358
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0607084104
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!

Ähnliche Einträge