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New Method for Evaluation of Genotoxicity, Based on the Use of Real-Time PCR and Lysogenic Gram-Positive and Gram-Negative Bacteria

A method for the detection of the SOS response as measured by the liberation of resident prophages from the genomes of their hosts is described. It is based on the use of two converging oligonucleotides that flank the attP attachment site of the phage as primers for real-time PCR. Amplification was...

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Main Authors: Soberón, Nora, Martín, Rebeca, Suárez, Juan E.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2007
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1892890/
https://ncbi.nlm.nih.gov/pubmed/17337549
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00407-07
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