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Substantial Background Reduction in Ligase-based Apoptosis Detection Using Newly Designed Hairpin Oligoprobes

A method for selective detection of apoptotic cells by ligation of DNA probes to the ends of specific double-stranded DNA breaks in tissue sections was recently reported (1). Unfortunately, this method produces high levels of background staining, due to nonspecific attachment of the probes to the ti...

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Main Authors: Didenko, Vladimir V., Boudreaux, Denise J., Baskin, David S.
格式: Artigo
語言:Inglês
出版: 1999
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC1892751/
https://ncbi.nlm.nih.gov/pubmed/10631490
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