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Imaging protein interactions with bioluminescence resonance energy transfer (BRET) in plant and mammalian cells and tissues

FRET is a well established method for cellular and subcellular imaging of protein interactions. However, FRET obligatorily necessitates fluorescence excitation with its concomitant problems of photobleaching, autofluorescence, phototoxicity, and undesirable stimulation of photobiological processes....

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Detalhes bibliográficos
Main Authors: Xu, Xiaodong, Soutto, Mohammed, Xie, Qiguang, Servick, Stein, Subramanian, Chitra, von Arnim, Albrecht G., Johnson, Carl Hirschie
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1891211/
https://ncbi.nlm.nih.gov/pubmed/17551013
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0701987104
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