Time-resolved x-ray diffraction reveals multiple conformations in the M–N transition of the bacteriorhodopsin photocycle

We measured the M–N transition of wild-type bacteriorhodopsin (pH 9, 10°C) by time-resolved x-ray diffraction study at SPring8 BL45XU-A. We confirmed the accumulation of M and N intermediates by absorbance measurements, and we found that the time resolution of x-ray diffraction experiments (244 ms)...

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Detalles Bibliográficos
Main Authors: Oka, Toshihiko, Yagi, Naoto, Fujisawa, Tetsuro, Kamikubo, Hironari, Tokunaga, Fumio, Kataoka, Mikio
Formato: Artigo
Idioma:Inglês
Publicado: The National Academy of Sciences 2000
Assuntos:
Biological Sciences
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC18909/
https://ncbi.nlm.nih.gov/pubmed/11106390
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