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Time-resolved X-ray diffraction reveals movement of F helix of D96N bacteriorhodopsin during M-MN transition at neutral pH.

D96N bacteriorhodopsin has two photointermediates with the deprotonated Schiff base: the M and MN intermediates. We measure the time-resolved x-ray diffraction of the D96N purple membrane after flash photoexcitation (pH 7.0, 25 degrees C). The data clearly show the M-MN transition during the D96N ph...

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Detalhes bibliográficos
Main Authors: Oka, Toshihiko, Yagi, Naoto, Tokunaga, Fumio, Kataoka, Mikio
Formato: Artigo
Idioma:Inglês
Publicado em: 2002
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1302049/
https://ncbi.nlm.nih.gov/pubmed/11964247
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