Active acetyl-CoA synthase from Clostridium thermoaceticum obtained by cloning and heterologous expression of acsAB in Escherichia coli
Acetyl-CoA synthase from Clostridium thermoaceticum (ACS(Ct)) is an α(2)β(2) tetramer containing two novel Ni-X-Fe(4)S(4) active sites (the A and C clusters) and a standard Fe(4)S(4) cluster (the B cluster). The acsA and acsB genes encoding the enzyme were cloned into Escherichia coli strain JM109 a...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
National Academy of Sciences
2000
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC18798/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11050160/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.220404397 |
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