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Active acetyl-CoA synthase from Clostridium thermoaceticum obtained by cloning and heterologous expression of acsAB in Escherichia coli

Acetyl-CoA synthase from Clostridium thermoaceticum (ACS(Ct)) is an α(2)β(2) tetramer containing two novel Ni-X-Fe(4)S(4) active sites (the A and C clusters) and a standard Fe(4)S(4) cluster (the B cluster). The acsA and acsB genes encoding the enzyme were cloned into Escherichia coli strain JM109 a...

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Autors principals: Loke, Huay-Keng, Bennett, George N., Lindahl, Paul A.
Format: Artigo
Idioma:Inglês
Publicat: The National Academy of Sciences 2000
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC18798/
https://ncbi.nlm.nih.gov/pubmed/11050160
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