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A rapid method for screening antimicrobial agents for activities against a strain of Mycobacterium tuberculosis expressing firefly luciferase.

We developed a rapid method to screen the efficacy of antimicrobial agents against Mycobacterium tuberculosis. A restriction fragment carrying a promoterless firefly luciferase gene was cloned into a 4,488-bp shuttle vector, pMV261, and luciferase was expressed under the control of a mycobacterial h...

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Detalhes bibliográficos
Publicado no:Antimicrob Agents Chemother
Principais autores: Cooksey, R C, Crawford, J T, Jacobs, W R, Shinnick, T M
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1993
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC187964/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8328785/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.37.6.1348
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