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Nick translation detection in situ of cellular DNA strand break induced by radiation.

DNA strand break in HeLa cells induced by radiation was detected using the in situ nick translation method. The cells were exposed to radiation of 3, 6, 12, 18, and 24 Gy in Lab-Tek tissue culture chamber/slides and were fixed with ethanol/acetic acid on the slide glass. The break sites in DNA were...

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Detalles Bibliográficos
Main Authors: Maehara, Y., Anai, H., Kusumoto, T., Sakaguchi, Y., Sugimachi, K.
Formato: Artigo
Idioma:Inglês
Publicado: 1989
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1879553/
https://ncbi.nlm.nih.gov/pubmed/2643885
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