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Optimizing PCR Positivity Criterion for Detection of Herpes Simplex Virus DNA on Skin and Mucosa

In 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA. Recently, we determined an optimal positivity criterion based on research specimens and a dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-fold lower than our previous cu...

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Main Authors: Magaret, Amalia S., Wald, Anna, Huang, Meei-Li, Selke, Stacy, Corey, Larry
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2007
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC1865863/
https://ncbi.nlm.nih.gov/pubmed/17329447
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01405-06
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