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Biochemical and genetic analysis of RNA cap guanine-N2 methyltransferases from Giardia lamblia and Schizosaccharomyces pombe

RNA cap guanine-N2 methyltransferases such as Schizosaccharomyces pombe Tgs1 and Giardia lamblia Tgs2 catalyze methylation of the exocyclic N2 amine of 7-methylguanosine. Here we performed a mutational analysis of Giardia Tgs2, entailing an alanine scan of 17 residues within the minimal active domai...

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Detalles Bibliográficos
Main Authors: Hausmann, Stéphane, Ramirez, Alejandro, Schneider, Susanne, Schwer, Beate, Shuman, Stewart
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2007
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1865056/
https://ncbi.nlm.nih.gov/pubmed/17284461
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl1150
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