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Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways

We demonstrate the efficacy of double-stranded RNA-mediated interference (RNAi) of gene expression in generating “knock-out” phenotypes for specific proteins in several Drosophila cell lines. We prove the applicability of this technique for studying signaling cascades by dissecting the well-characte...

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Detalhes bibliográficos
Main Authors: Clemens, James C., Worby, Carolyn A., Simonson-Leff, Nancy, Muda, Marco, Maehama, Tomohiko, Hemmings, Brian A., Dixon, Jack E.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2000
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC18635/
https://ncbi.nlm.nih.gov/pubmed/10823906
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